Plasmid

Part:BBa_K3819022:Design

Designed by: Luo SongWen   Group: iGEM21_UESTC-China   (2021-09-28)


Lipase plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 727
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have adopted strategies such as codon optimization to change the restriction site in order to be compatible with RFC.


Source

1 GAP promoter (BBa_K3819009) 2 alpha-factor（BBa_K3105674） 3 SMT3 (BBa_K3819005) 4 lipase (BBa_K3819128) 5 Myc tag

References